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Journal: bioRxiv

Article Title: HAPI Cells are SIM-A9-related Mouse Microglial Cells Useful for In Vitro Modeling of Microglial Immunometabolism

doi: 10.64898/2026.02.11.705385

Figure Lengend Snippet:

Article Snippet: The number of repeats varies greatly among cell lines, making STRs highly useful for identification by providing a cell line “fingerprint.” STR profiling revealed that HAPI cells are a 100% STR match for SIM-A9 cells, a mouse microglial cell line in the ATCC database that was reported to originate from a postnatal day 1 primary mouse microglial culture through spontaneous immortalization ( ) ( ; Figure S1).

Techniques:

HAPI cells express microglia-selective markers. (A) Representative image of HAPI cells immunostained for TMEM119. (B) Detection of Tmem119 polymerase chain reaction (PCR) product in sample prepared from HAPI cells, SIM-A9 cells, mouse cortex tissue, mouse bone marrow-derived macrophages (BMDMs), or Neuro2a cells. An arrow designates the band corresponding to the predicted product size of 119 base pairs (bp). (C) Detection of Cx3cr1 PCR product in the same samples evaluated in B. An arrow designates the band corresponding to the predicted 109 bp product.

Journal: bioRxiv

Article Title: HAPI Cells are SIM-A9-related Mouse Microglial Cells Useful for In Vitro Modeling of Microglial Immunometabolism

doi: 10.64898/2026.02.11.705385

Figure Lengend Snippet: HAPI cells express microglia-selective markers. (A) Representative image of HAPI cells immunostained for TMEM119. (B) Detection of Tmem119 polymerase chain reaction (PCR) product in sample prepared from HAPI cells, SIM-A9 cells, mouse cortex tissue, mouse bone marrow-derived macrophages (BMDMs), or Neuro2a cells. An arrow designates the band corresponding to the predicted product size of 119 base pairs (bp). (C) Detection of Cx3cr1 PCR product in the same samples evaluated in B. An arrow designates the band corresponding to the predicted 109 bp product.

Article Snippet: The number of repeats varies greatly among cell lines, making STRs highly useful for identification by providing a cell line “fingerprint.” STR profiling revealed that HAPI cells are a 100% STR match for SIM-A9 cells, a mouse microglial cell line in the ATCC database that was reported to originate from a postnatal day 1 primary mouse microglial culture through spontaneous immortalization ( ) ( ; Figure S1).

Techniques: Polymerase Chain Reaction, Derivative Assay